【摘要】 目的探讨缺血性卒中后外周血CD34+细胞和脑组织AC133抗原的变化。方法本研究采用大鼠大脑中动脉缺血再灌注线栓法模型,缺血2h。将成年SD雄性大鼠随机分为正常组、假手术组、动物模型组,分别取缺血再灌注1、3、6、12、24、48、72h、4、7、14d等作为观察点。取再灌注1、3、6、12、24、48、72h、4、7、14d外周血标本,采用流式细胞术检测外周血单个核CD34+细胞平均荧光强度。采用免疫组化染色法检测2、3、4、7d脑组织AC133抗原表达。每个观察点5只大鼠,共60只大鼠,根据神经功能计分纳入实验。结果①大鼠脑缺血/再灌注3d外周血单个核CD34+细胞平均荧光强度明显降低,直到7d。14d恢复到基线水平。②AC133抗原在再灌注4d梗死半球半暗带区域的血管内皮细胞表达阳性,3、7d组无阳性表达。结论脑缺血再灌注后早期外周血CD34+干细胞明显下降,脑梗死半暗带区域部分血管内皮细胞出现AC133抗原的表达,提示内源性血管内皮干细胞可能参与脑缺血损伤的血管修复。更多还原

【Abstract】 Objective To studied roles of circulating CD34+ monocytes and AC133+ cells in rats after focal cerebral ischemia/reperfusion.Methods Right middle cerebral artery suture occlusion model was used.60 adult SD male rats were randomly divided into 3 groups.They were control,sham-control and animal models which included 1、3、6、12、24h,2、3、4、7、14d reperfusion groups.After right middle cerebral artery occluded,the rats were suffered from ischemia for 2 hours,and then reperfusion was allowed for different time as mentioned above before decapitation.Circulating CD34+ cells were detected by cytoflowmetric analysis and cerebral AC133 antigen expression in 2、3、4、7d reperfusion groups were tested by immunohistochemistry.Results ①Circulating CD34+ cells sharphy decreased from 3 to 7 days and returned to basal values on the 14th day.②AC133 antigen expression was only observed in endothelial cells in areas around infarct core in 4 days.Conclusion The numbers of circulating CD34+ progenitor cells and the expression of AC133 antigen was changed according to different time point after focal cerebral ischemia/reperfusion in rat.It may be suggested that endothelial progenitor cells had taken part in cerebral vascular repair in rat MCAO models.更多还原