【Abstract】 The allotetraploid in cross-fertilized eggs of Chlamys farreri♀× Patinopecten yessoensis♂ was induced in this paper.In order to find out the appropriate duration time of 6-DMAP treatment,ploidy status of induction offspring was analyzed by flow cytometry.The results showed that the optimal duration time of 6-DMAP treatment was to treat the fertilized eggs with 50 mg/L 6-DMAP for 15 min based on the integrated evaluation of the results of fertilization rate,survival rate of D-larvae,development status of larvae,and percentage of tetraploid.The results of flow cytometry showed that 17.3% tetraploid was obtained which showed that allotetraploid could be gotten with this method.The causes of the problems in the work were also discussed in this paper.更多还原