【摘要】 采用单因子试验设计方法,进行了饲料中添加维生素E对花鲈生长(增重率、存活率、特定生长率、饲料效率)、组织中维生素E积累量和免疫指标(血清中溶菌酶活性和总补体活性)影响的研究,设计了5个不同维生素E水平(0.6、24.7、49.0、98.8、198.2 mg/kg)的等氮等能饲料,对花鲈进行为期8周的生长实验,每个水平含3个重复,每个养殖单元放养初始体重为10.1 g左右的花鲈20尾。饲养试验在海水网箱(1.5 m×1.0 m×1.0 m)中进行。结果表明:饲料中未添加维生素E组的增重率、饲料效率、特定生长率以及存活率显著低于维生素E添加组,随着饲料中维生素E添加量由0.6提高到49.0 mg/kg时,花鲈的增重率和特定生长率显著提高,以增重率和特定生长率为指标,花鲈最佳生长性能的饲料维生素E添加量为60.5mg/kg左右;对鱼体生物学指标的分析表明,饲料中维生素E添加量达到49 mg/kg时能显著影响鱼体的肝体比指数,鱼体水分、粗脂肪不受饲料中维生素E添加水平的影响,然而对鱼体粗蛋白含量的影响显著;随着饲料中维生素E添加量由0.6提高到49.0 mg/kg时,花鲈肌肉和肝脏中维生素E的积累量显著升高,但当维生素E添加量达到49.0 mg/kg后,肌肉和肝脏中维生素E的积累量变化不显著;饲料中未添加维生素E组的花鲈血清溶菌酶和总补体活性显著低于维生素E添加组,花鲈血清溶菌酶和总补体活性随着维生素E添加量的增加而显著升高,饲料维生素E添加量达到98.8 mg/kg时,血清溶菌酶和总补体活性较高。综上所述,花鲈生长和免疫的维生素E适宜添加量为60.5~98.8 mg/kg。更多还原

【Abstract】 An experiment was conducted to study the effects of dietary vitamin E on growth(weight gain rate,feed efficiency ratio,specific growth rate and survival rate),vitamin E concentration in tissue and immune response(lysozyme activity and total complement activity)of Japanese seabass(Lateolabrax japonicus).Five iso-nitrogenous and iso-energetic diets were formulated to contain graded level of vitamin E(0.6,24.7,49.0,98.9,and 198.2 mg/kg diet,respectively) and fed to 15 floating netcages(1.5 m×1.0 m×1.0 m)with 20 fish(initial weight: 10.1 g) each twice daily(09:30,16:30)for 8 weeks.Results indicated that after 8 weeks of the feeding trial,weight gain rate,feed efficiency ratio,specific growth rate and survival rate of the fish fed with the control diet were significantly lower than those with vitamin E supplemented diets.The weight gain rate and specific growth rate increased significantly with increasing dietary vitamin E from 0.6 to 49.0 mg/kg and the optimal vitamin E content for maximum growth is about 60.5 mg;Moisture and fat concentration in whole body of Japanese seabass were not affected by the supplemental levels of dietary vitamin E,but the protein concentration increased significantly with increasing dietary vitamin E from 0.6 to 49.0 mg/kg.Meanwhile,the vitamin E concentration in liver and musle increased significantly with increasing dietary vitamin E from 0.6 to 49.0 mg/kg,but no significant differences were observed among fish fed the diets with equal to or higher than 49.0 mg/kg of vitamin E.The serum lysozyme activity and total complement activity of the fish fed with the control diet were significantly lower than those with vitamin E supplemented diets,the serum lysozyme activity and total complement activity of Japanese seabass increased significantly with increasing dietary vitamin E supplement,and the Japanese seabass had the maximal level of lysozyme activity and total complement activity when fed 98.8 mg/kg.Based on the information above,the optimal dietary vitamin E level for optimum growth performance and immune responses in Japanese seabass is from 60.5 to 98.8 mg/kg.更多还原