【摘要】 本研究以石楠为受体材料,利用农杆菌浸染方法开展沙冬青抗寒基因AmGS转化试验,通过对不同的受体材料浸染部位、菌液浓度、乙酰丁香酮和浸染时间对转化材料的死亡率%、分化率%、分化芽数(个)、芽苗长度(cm)以及芽苗颜色的分析,得出最佳转化方法为:采用茎尖为转化材料,菌液浓度为2/3~1/2原液,浸染时间为10 min,添加乙酰丁香酮浓度为25~30 mg/L。其中浸染部位是影响基因高效转化的最主要因素,菌液浓度、乙酰丁香酮和浸染时间的作用次之,经在50 mg/L浓度的卡那霉素培养基筛选培养,获得一批的抗性植株,经PCR检测获得6株转化株系。更多还原

【Abstract】 By means of Agrobacterium contamination,cold-tolerant gene AmGs,absorbed form Ammopiptanthus mongolicus,was transformed into Chinese photinia.We made an investigation what different contamination position,Agrobacterium concentration,acetosyringone and contamination time of the acceptor material affected the transformant death rate,differentiation rate,number of differential buds,buds length and buds color.Then we got the best transformation method that Agrobacterium was 2/3to 1/2 as dense as original liquid,acceptors were contaminated for 15 minutes and acetosyringone was 25 mg/L to 30 mg/L when stem apexes were transformed.Contamination position was the major factor of effects on the gene advanced transformation.The effects of bacterial concentration and contamination time were minor factors.A group resistant plants wene obtained through with the oulture medium contains 50 mg/L kanamycin,then six transferplants can he got by PCR examination.更多还原