【摘要】 目的研究防己(Stephenia tetrandra S Moore)对小鼠胸腺的细胞DNA的影响及其作用机制。方法取小鼠胸腺的细胞,随机分为对照组和药物组,药物组又再分为两组(防己煎剂浓度分别为10μg/ml,100μg/ml),采用DNA凝胶电泳观察其对小鼠胸腺细胞的作用。结果防己煎剂(10μg/ml、100μg/ml)对胸腺的细胞分别处理12h后,可见典型的DNA梯形条带。结论防己煎剂可诱导小鼠胸腺的细胞凋亡。更多还原

【Abstract】 Objective To investigate the effect of Han-Fang-Chi/Stephenia tetrandra S Moore on the thymic cell.Methods Thymic cells of trial mice were respectively divided into control group and treated groups.The-treated groups were subdivied into the 10 μg/ml goup and 100 μg/ml group.The DNA of thymic cells was detected by DNA gel electrophoresis.Results When the cells were treated by Han-Fang-Chi for 12 h,the typical "DNA ladder" was observed.Conclusion Han-Fang-Chi decoction can induce the apoptosis of thymic cells in mice.更多还原