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不同金银花种质资源ISSR遗传多样性研究
Studies on genetic diversity of Lonicera(family Caprifoliaceae) based on ISSR marker
【摘要】 应用ISSR标记技术,对来自国内忍冬属(Lonicera Linn.)的19个居群进行遗传多样性和遗传结构的分析.从100条ISSR随机引物中筛选出19条引物对19个忍冬居群进行扩增,共得到305条清晰的扩增位点,其中多态性位点273个,多态位点百分率(PPB)为89.51%.遗传分化系数(Gsr)为0.5624,这显示19个居群的分子变异主要存在于居群间.
【Abstract】 Inter-simple sequence repeats (ISSR) markers were employed to assess genetic diversity and population structure in nineteen populations of Lonicera Linn.,a source of anti-inflammatory herbal medicine.Using 19 screened primers,305 discernible DNA fragments were generated of which 273 (89.51%) were polymorphic.The coefficient of gene differentiation (GST=0.5624) showed that the total molecular variance of 19 populations was mainly existed among populations.
【关键词】 遗传多样性;
ISSR;
忍冬;
金银花;
药用植物;
【Key words】 genetic diversity; ISSR; Lonicera; FlosLonicerae; medical plant;
【Key words】 genetic diversity; ISSR; Lonicera; FlosLonicerae; medical plant;
【基金】 四川省育种工程青年基金(2007)
- 【文献出处】 四川大学学报(自然科学版) ,Journal of Sichuan University(Natural Science Edition) , 编辑部邮箱 ,2009年06期
- 【分类号】S567.79
- 【被引频次】16
- 【下载频次】65