节点文献
IL-21/4-IgG4融合基因的真核表达
Construction and expression of eukaryotic expression vector containing IL-21/4-IgG4 fusion protein
【摘要】 利用新型细胞因子 IL-21的变异体 IL-21/4与 IgG4构成融合基因IL一21/4一IgG4.将构建好的融合基因载体 poptivec-IL-21/4-IgG4转染到中华仓鼠卵巢细胞中,成功并稳定表达了 IL-21/4-IgG4融合蛋白.
【Abstract】 IL-21/4 is the mutant of a new-found cell factor.It was bonded to IgG4 to form a fusion gene IL-21/4-IgG4 in the vector poptivec-IL-21/4-IgG4,which was then transfected into Chinese Hamster Ovary (CHO) cells.It was demonstrated that the fusion protein could be stably expressed in CHO cells.
【关键词】 CHO 细胞;
真核表达;
转染;
IL-21;
融合基因;
【Key words】 CHO cells; eukaryotic; expression; transfetion; fusion gene; IL-21;
【Key words】 CHO cells; eukaryotic; expression; transfetion; fusion gene; IL-21;
【基金】 国家自然科学基金(30371307)
- 【文献出处】 四川大学学报(自然科学版) ,Journal of Sichuan University(Natural Science Edition) , 编辑部邮箱 ,2009年04期
- 【分类号】Q78
- 【下载频次】13