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微生物发酵转化黄芩苷生成黄芩素的研究

The studies on the transformation from baicalin into baicalein by the microbial transformation

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【Author】 WANG Hong~1 , GAO Pei~1 , LIAO Yong~2 , WANG Jing~1 , SUN Qi-Ling~1(1. Key Laboratory of Bio-resources and Eco-environment of Ministry of Education,College of Life Sciences, Sicbuan University, Chengdu 610064, China;2. Department of Equipment, Sichuan University, Chengdu 610064, China)

【机构】四川大学生命科学学院生物资源与生态环境教育部重点实验室；四川大学设备处；

【摘要】筛选到一株产β-葡萄糖醛酸酶的菌株HQ-10,能够以黄芩为底物,通过发酵将黄芩中的黄芩苷转化为黄芩素。通过对发酵条件进行优化,黄芩苷转化率近于92%。发酵产物经TLC、HPLC、质谱等方法检测确定为黄芩素。更多还原

【Abstract】 A strain Aspergillus niger (HQ-10) was screened with the capability of transformation from baicalin into baicalein which was determined. The parameters of fermentation and medium were optimized. In the optimum condition of fermentation, the rate of transformation was about 92%. The product of transformation was determined by TLC, HPLC and the mass spectrogram.更多还原

【关键词】微生物转化； β-葡萄糖醛酸酶；黄芩苷；黄芩素；
【Key words】 microbial transformation； β-glucuronidase； baicalin； baicalein；

【基金】四川省中医药管理局科技项目(200824)

【文献出处】四川大学学报(自然科学版) ,Journal of Sichuan University(Natural Science Edition) , 编辑部邮箱 ,2009年03期

【分类号】TQ461
【被引频次】29
【下载频次】322

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