【摘要】 采用气相色谱/质谱(GC/MS)联用的方法,对林麝麝香中的甾体成分进行分析,确定了林麝麝香样品含有多种甾体成分的结构,并分析了不同来源的林麝麝香的麝香酮及甾体成分。通过检索NIST05质谱库,进一步确定了麝香中含有16种甾体成分。利用外标法、标准曲线法同时测定了麝香样品中3种甾类成分(胆固醇、苯胆烷醇酮及麝香酮)的含量,麝香酮的定量分析显示所有样品麝香中麝香酮含量均较高(30.1～45.2mg/g),但甾类成分含量波动较大。聚类分析显示,9个麝香样品聚为两支。利用GC/MS技术检测麝香成分的方法,可以提供麝香较为全面的甾类信息,可高效准确地对麝香进行质量分析。更多还原

【Abstract】 Musk is one of the precious animal products used in traditional Chinese medicine.Musk samples were analyzed by gas chromatography/mass spectrometry(GC/MS)in this study.It was found that musk contained a complex mixture of different steroids.The steroidal components of different samples were also analyzed and compared.Sixteen kinds of steroids in musk samples were confirmed by NIST05 library searching.Three pharmacologically active components(muscone,cholesterol,and etiocholan-3α-ol-17-one)were quantified in the different samples.The results showed that changes in cholesterol and etiocholan-3α-ol-17-one were obvious in all musk samples.In contrast,muscone levels in musk samples were stable(30.1～45.2 mg/g).In order to distinguish between musk samples,a hierarchical cluster analysis was performed.The results showed that the 9 musk samples could be divided into two groups.With the help of gas chromatography-mass spectrometry,the quality of musk samples can be analyzed quickly and accurately.更多还原