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饲料维生素E水平对黑鲷抗氧化能力和鱼肉货架期的影响

Effects of Vitamin E Supplementation in Preslaughter Diet on Antioxidant Enzyme Activities and Fish Shelf Life of Sparus macrocephalus

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【作者】 朱勇飞吴天星张晓东

【Author】 ZHU Yong-fei1,2,WU Tian-xing3,ZHANG Xiao-dong1(1.School of Animal Sciences,Zhejiang University,Hangzhou 310027,China;2.Key Laboratory for Molecular Design and Nutrition Engineering,Ningbo Institute of Technology,Zhejiang University,Ningbo 315100,China;3.Department of Chemistry,Zhejiang University,Hangzhou 310027,China)

【机构】 浙江大学动物科学学院浙江大学宁波理工学院浙江大学理学院

【摘要】 探讨了饲料维生素E水平对黑鲷抗氧化酶活力和肉品质的影响。300尾平均初重为(350±12)g黑鲷随机分成3组,放入9个网箱。饲料中添加3个水平的维生素E(250、500和1000mg/kgα-生育酚乙酸酯),饲养8周。结果发现:(1)随着饲料中维生素E添加量的增加,黑鲷血清SOD、CAT酶活力明显上升,其中VE1000组最高,VE1000和VE500组显著高于VE250组(P<0.05);(2)饲料维生素E含量对血清中谷胱甘肽过氧化物酶(GPX)活力没有显著影响(P>0.05);(3)鲜活黑鲷肌肉氧化代谢产物丙二醛(MDA)的含量3个组间没有显著差异(P>0.05);(4)黑鲷冷藏第3、6、9天,高水平维生素E(VE500和VE1000)组肌肉MDA含量显著低于低水平维生素E组(V250)(P<0.05)。

【Abstract】 The effects of dietary-tocopheryl acetate supplementation on antioxidant enzyme activities and fillet quality in commercial-size Sparus macrocephalus were studied.Three hundred fish with initial average weight of(350±12)g were divided into three groups(VE 250,VE 500 and VE 1 000)and reared in 9 cages.The fish were fed for 8 weeks with three diets containing different levels of dietary-tocopheryl acetate(250,500 and 1 000 mg/kg).The results show that:(1)Serum superoxide dismutase(SOD)and catalase(CAT)activity had obviously increased with increase of dietary vitamin E levels.VE 1 000 is the lowest group,VE 1 000 and VE 500 were significantly lower than the VE 250 group(P<0.05);(2)Supplemention of dietary vitamin E had no significant impact on serum Glutathione peroxidase(GPX)of Sparus macrocephalus(P>0.05);(3)Muscle malondialdehyde(MDA)levels did not change significantly(P>0.05)among three groups of fresh fish;(4)The level of muscle MDA was significantly(P<0.05)lower in the groups fed high α-tocopheryl acetate supplemented diets(VE 500 and VE 1 000)than those receiving low level of α-tocopheryl acetate(VE 250)on days of 3th,6th and 9th in the refrigeration process.

【基金】 浙江省科技厅重大农业科技攻关项目(No.2006c12098)
  • 【文献出处】 水生态学杂志 ,Journal of Hydroecology , 编辑部邮箱 ,2009年03期
  • 【分类号】S963
  • 【被引频次】12
  • 【下载频次】248
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