【摘要】 从正常人血液中提取人基因组DNA。以其为模板,扩增人α-乳白蛋白(α-LA)2.36kb的总DNA序列,经测序鉴定后,将其连接到PSV载体SV40启动子后,构建真核表达载体LA-DNA-psv.扩增牛XS1-酪蛋白5’调控序列约1.2kb的片段,测序鉴定。切去LA-DNA-psv载体原来的SV40启动子,连接牛αS1-酪蛋白5’调控序列约1.2kb的片段为启动子,构建真核表达载体αS1-LA-DNA-psv。为在动物乳腺中特异高效表达人α-乳白蛋白做准备研究。更多还原

【Abstract】 Human genomic DNA was extracted from normal human blood.Human alpha lactalbumin DNA Sequence which is 2.36kb was cloned and sequenced.Then the sequence was linked to PSV vector and the expression vector named LA-DNA-psv was constructed.The 1.2kb region of 5’flanking sequence of bovine αS1 casein gene was cloned, sequenced and combined with the LA-DNA-psv vector which was cut off SV40 promotor.The expression vector named αS1-LA-DNA-psv was constructed.This study was done to prepare to express human alpha lactalbumin in animal mammary gland.更多还原