【摘要】 【目的】建立新生大鼠大脑皮质星形胶质细胞体外培养的方法。【方法】选用出生后24h内的SD大鼠,分离大鼠大脑皮质细胞,加入含体积分数10%胎牛血清的高糖DMEM培养基培养,于倒置相差显微镜下观察细胞生长形态;取传代培养的第3代细胞,采用计数板计数法和四甲基偶氮唑盐(MTT)法检测细胞生长情况。【结果】从新生大鼠大脑皮质分离的细胞生长旺盛,星形胶质细胞形态典型,未见其他种类细胞生长。【结论】建立了一种简单易行的大鼠星形胶质细胞体外培养方法。更多还原

【Abstract】 Objective To establish a method for the in-vitro culture of astrocytes from cerebral cortex of neonatal rats.Methods Astrocytes were isolated from cerebral cortex of the neonatal SD rats aged 24 hours,and then were cultured in the high-glucose DMEM medium with 10% fetal bovine serum added.The morphological features of astrocytes were examined under inverted microscope.The growth of the third generation of astrocytes was observed with counting plate method and methyl thiazolyl tetrazolium (MTT) assay.Results The astrocytes from cerebral cortex of neonatal rats grew well,the astrocytes showed typical morphological features,and no other kinds of cells was found.Conclusion A simple and practical in-vitro culture method for rats astrocytes is established.更多还原