【摘要】 采用外加代谢酶抑制剂阻断法,对PAL酶专一性抑制剂(AOA)和4CL酶专一性抑制剂(MDCA)处理后野葛愈伤异黄酮的生物合成,以及预苯酸、对羟基苯丙酮酸和对羟基苯丙乳酸的积累情况进行研究。结果表明,AOA处理虽显著抑制以苯丙氨酸为前体的异黄酮化合物的合成,但在野葛愈伤中仍有异黄酮的显著积累,说明野葛愈伤培养体系中应该存在其他黄酮生物合成途径。MDCA处理的野葛愈伤组织中,预苯酸、对羟基苯丙酮酸和对羟基苯丙乳酸发生了显著积累,表明上述物质可能为异黄酮生物合成的前体化合物。更多还原

【Abstract】 The specific inhibitor of phenylalanine ammonia lyase,α-aminooxyacetic acid(AOA),and that of 4-coumarate-CoA ligase,3,4-(methylenedioxy)-cinnamic acid(MDCA),were applied in the block experiments for isoflavone biosynthesis,and their effects on isoflavone biosynthesis and the accumulation of prephenate,p-hydroxyphenylpyruvate and p-hydroxyphenyllactate in calli of kudzu(Pueraria lobata(Willd) Ohwi) were examined,respectively.The results showed that isoflavonoid biosynthesis from the pathway using L-phenylalanine as the precursor was inhibited after AOA treatment,but the isoflavone content still increased significantly in the kudzu callus,which suggested that another pathway or other pathways of isoflavone biosynthesis should exist in the kudzu callus.The significant accumulation of prephenate,p-hydroxyphenylpyruvate and p-hydroxyphenyllactate in the kudzu callus with MDCA treatment showed that these chemicals can serve as possible precursors for isoflavone biosynthesis.These results can provide a new evidence for enzymological research to verify the novel possible pathway from prephenate,p-hydroxyphenylpyruvate through p-hydroxyphenyllactate to p-coumaric acid.更多还原