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橡胶草的组织培养研究
Tissue Culture of Taraxacum kok-saghyz Rodin
【摘要】 以橡胶草的茎叶为外植体,以MS为基本培养基,探索了橡胶草组织培养的最佳途径。结果表明:茎叶愈伤诱导的最佳培养基为MS+6-BA2.0mg/L+2-4,D1.0mg/L;最佳分化培养基为MS+6BA0.8mg/L+NAA0.3mg/L;诱导生根的最佳培养基是1/2MS(蔗糖减半)+NAA0.5mg/L。
【Abstract】 Stems and leaves of rubber dandelion(Taraxacum kok-saghyz Rodin) were used as explants for tissue culture,with MS medium as the basic medium.The results showed that the optimum cultural mediums for tissue culture of explants of stems and leaves were MS + 6-BA 2.0 mg/L+ 2-4,D1.0 mg/L for callus inducing MS + 6BA0.8 mg/L + NAA0.3 mg/Lfor differentiation,and 1/2MS + NAA0.5 mg/L for rooting.
【基金】 橡胶所基本业务专项(NO.xjszx_06)
- 【文献出处】 热带农业工程 ,Tropical Agricultural Engineering , 编辑部邮箱 ,2009年04期
- 【分类号】S576
- 【被引频次】24
- 【下载频次】335