【摘要】 目的探讨乙型肝炎患者HBV-DNA含量与血清免疫学标志物、肝功能的关系。方法将362例乙肝患者按血清免疫学标志物不同模式分成两组:HBeAg阳性组,82例;HBeAg阴性组,280例。HBV-DNA、免疫学标志物和肝功能检测分别采用荧光定量PCR法、酶联免疫吸附法和生化分析法,数据分析采用SPSS11.0统计学软件。结果HBeAg阳性组HBV-DNA含量≥103IU/mL者为97.56%,高于HBeAg阴性组的43.57%,二者间差异有统计学意义(χ2=74.96,P<0.01)。HBV-DNA含量103～104IU/mL者,HBeAg阳性组为2.44%,低于HBeAg阴性组的35.00%,二者间差异有统计学意义(χ2=33.63,P<0.01)。HBV-DNA含量为105～106IU/mL者,肝功能异常者的比例为64.70%。结论HBeAg阳性者乙肝病毒复制水平较高,HBeAg阴性者病毒复制水平相对较低,但病毒并非停止复制,HBeAg阴性者也存在高水平病毒复制。判断乙型肝炎患者是否存在病毒复制,是否具有传染性,最直接、最可靠的指标是HBV-DNA定量检测。更多还原

【Abstract】 Objective To determine the correlation between the HBV-DNA expression and serum immunological markers and liver function of hepatitis B patients.Methods 362 cases were divided into HBeAg-positive and HBeAg-negative group.HBV-DNA and the immunological markers were determined by RT-PCR and ELISA, respectively.Liver function was evaluated by biochemical analytical method.Statistical analysis was performed using SPSS11.0 software.Results Compared with the HBeAg-negative group(43.57% , χ2=74.96, P<0.01), 97.56% of HBeAg-positive patients were found to have a level of 103 IU / ml HBV-DNA.In HBeAg-positive group, the percentage of patients(2.44%) with 103～104 IU / mL of HBV-DNA was lower than the HBeAg-negative group(35%, χ2=33.63,P< 0.01).64.7% of patients with HBV-DNA concentration of 105～106 IU / mL had liver dysfunction.Conclusion The expression level of HBV-DNA in HBeAg-positive group was high.The low level of expression of HBV-DNA in HBeAg-negative group could not truly reflect the status of HBV replication.Quantitative measurement of HBV-DNA is an appropriate method to evaluate the stautus of hepatitis B infection.更多还原