【摘要】 目的观察脂多糖(lipopolysaccharide,LPS)诱导家蝇胚胎细胞产生抗菌肽的抗菌活性。方法LPS诱导家蝇胚胎细胞系(MDEC-07114),收集诱导后4、8、12、16、20、24h上清液,三氯醋酸提取抗菌肽,抑菌圈法测定抗菌活性。结果LPS作用处于对数生长的家蝇胚胎细胞后,细胞形态、增殖无明显变化;诱导后8h家蝇胚胎细胞开始产生抗菌肽,16h达高峰,诱导后24h仍有抗菌活性。结论LPS可诱导家蝇胚胎细胞系MDEC-07114产生具抗菌活性的抗菌肽。更多还原

【Abstract】 Objective To examine the antibacterial activity of antibacterial peptides produced from the cultural embryonic cell of the Musca domestica induced by lipopolysaccharide(LPS).Methods Embryonic cell lines of the Musca domestica(MDEC-07114) were induced with LPS.Culture supernatant was collected at 4,8,12,16,20 and 24 h after induction.The antibacterial peptides were recovered by trichloroacetic acid precipitation method,and the antibacterial activity was determined using the zone inhibition assay.Results Embryonic cell at the logarithmic growth phase was induced by LPS.There was no significant change in the cell number and the cell morphology.Antibacterial peptides was produced at 8 h and reached a peak level at 16 h after stimulation.The antibacterial activity could still be detected at 24 h after the treatment.Conclusion Antibacterial peptides can be produced by the LPS-treated embryonic cell lines of the Musca domestica(MDEC-07114).更多还原