【摘要】 目的探讨转录因子SP-1在肿瘤坏死因子TNFα诱导血管内皮细胞血红素氧化酶HO-1基因表达中的作用。方法用ELISA法检测HO-1的蛋白表达,用Westernblot法检测SP-1的蛋白水平。结果TNFα(200U/ml)处理24h后,HUVEC胞内HO-1和AP-1蛋白水平均明显升高;NAC(20mmol/L)预处理可明显抑制胞浆内AP-1及HO-1的蛋白含量(P<0.05)。结论SP-1部分介导了TNFα对HO-1基因表达的诱导作用。更多还原

【Abstract】 Objective To investigate the roles of specificity protein-1(SP-1)on tumor necrosis factor-α(TNFα)-induced expression of HO-1 in endothelial cells.Methods The expressions of the HO-1 and SP-1 at the protein were measured by ELISA and western blot.Results TNFα(200U/ml)treatment caused an increase in the protein contents of the HO-1 and SP-1,which were markedly reduced by pretreatment with NAC(20mmol/L).Conclusions SP-1 may play a major role in the TNFα-induced upregulation of HO-1 gene.更多还原