【摘要】 目的:研究食管鳞癌组织中Survivin mRNA与蛋白表达的相互关系,探讨两者在食管鳞癌发生和发展中的作用。方法:采用原位杂交技术检测30例食管鳞癌及相应的癌旁组织中Survivin mRNA的表达,同时采用免疫组织化学SP法检测相应标本中Sur-vivin蛋白的表达。结果:在食管鳞癌和相应的癌旁组织中Survivin mRNA表达的阳性率分别为66.7%和10.0%,Survivin蛋白表达的阳性率分别为76.7%和16.0%。食管鳞癌组远高于癌旁组,差异有统计学意义,P<0.01。Survivin mRNA与蛋白在食管鳞癌组织中的阳性表达具有一致性(P>0.05),其阳性率高低与性别、年龄、肿瘤大小无关,而与组织学分级、淋巴结转移、TNM分期有关。结论:Sur-vivin基因的异常表达可能对食管鳞癌的发生和发展起重要作用。更多还原

【Abstract】 OBJECTIVE:To investigate the expression of Survivin mRNA and protein in esophageal squamous carcinoma(ESC) tissues and its correlationship with clinical data.METHODS:In 30 cases of ESC,the expression of Survivin mRNA was detected by in situ hybridization(ISH),and the expression of Survivin protein by immunohistochemical method and SP technique in cancerous tissues and paraneoplastic tissues.RESULTS:In cancerous tissues and paraneoplastic tissues of ESC,the positive expression of Survivin mRNA was 66.7% and 10.0% respectively,and the positive expression of Survivin protein was 76.7% and 16.0% respectively,showing significant difference(P<0.01).The positive expression of Survivin mRNA in ESC tissues was similar to that of Survivin protein(P>0.05),which was not related to sex,age and the size of tumor,but related to histological grade,lymphatic metastasis and TNM staging.CONCLUSION:The abnormal expression of Survivin gene may play an important role in the genesis and development of ESC.更多还原