【摘要】 目的:检测食管癌组织及其区域淋巴结中树突状淋细胞(DC)及Th1/Th2细胞因子的表达,探讨食管癌发生及与免疫逃逸的关系。方法:采用流式细胞术检测57例食管癌组织、癌旁组织,16例食管炎性组织,62枚区域淋巴结中DCCD1a、CD83及共刺激因子CD80、CD86的表达,ELISA法检测组织匀浆中Th1型细胞因子IL-2、IFN-γ,Th2型细胞因子IL-4、IL-10的水平。结果:57例食管癌组织中CD1A、CD83、CD80及CD86的表达分别为7.34±1.59、2.87±0.55、5.75±1.06和27.34±7.21,显著低于炎性、正常和癌旁组织;癌组织中IL-2、IFN-γ表达水平分别为8.94±1.72、9.34±2.16,低于炎性、正常和癌旁组织;IL-4、IL-10的水平分别为5.24±0.35、5.3±1.02,高于炎性、正常和癌旁组织(P<0.05,P<0.01)。区域淋巴结组织中CD1A、CD83、CD80及CD86的表达在有转移癌者明显低于无转移癌者;IL-2、IFN-γ表达水平降低;IL-4、IL-10的水平增高,差异有统计学意义(P<0.05,P<0.01)。结论:食管肿瘤组织和区域淋巴结内DC的减少及Th1/Th2失调,可能是食管肿瘤发生免疫逃逸的原因。更多还原

【Abstract】 OBJECTIVE:To detect the expressions of dendritic cells and Th1/Th2 cytokines in esophageal carcinoma and its reginal lymph node.METHOD:The expressions of CD1a,CD83,CD80 and CD86 in 57 tumor tissues and paratumor tissues,16 inflammatory tissues and 62 regional lymph nodes were determined by using flow cytometry.Th1 cytokines IL-2 and IFN-γ,Th2 cytokines IL-4 and IL-10 were tested in the tissue homogenate.RESULTS:The expressions of CD1a,CD83,CD80 and CD86 in tumor were 7.34±1.59,2.87±0.55,5.75±1.06 and 27.34±7.21 respectively,and lower than those in paratumor tissues and inflammatory tissues.The levels of IL-2 and IFN-γ were also low in tumor compared with those in paratumor tissues and inflammatory tissues with 8.94±1.72 and 9.34±2.16,respectively.The levels of IL-4 and IL-10 in tumor were 5.24±0.35 and 5.3±1.02,higher than those in other tissues(P<0.05,P<0.01).In metastatic lymph nodes,the expressions of CD1a,CD83,CD80 and CD86,as well as the levels of IL-2 and IFN-γ,decreased significantly compared with those in metastases zero lymph nodes.On the other hand,the levels of IL-4 and IL-10 increased significantly in metastatic lymph nodes(P<0.05,P<0.01).CONCLUSION:The decreased expression of dentric cells and imbalance of Th1/Th2 cytokines in esophageal carcinoma and its reginal lymph node may be one of the mechanisms of immune escape of tumor cells.更多还原