【摘要】 目的:观察罗勒胶囊对大鼠移植性肝癌TACE术后肿瘤组织中HIF-1α、MMP-2、MMP-9及血清AFP表达的影响。方法:建立大鼠移植性肝癌模型,造模成功后随机分为非TACE对照组、TACE+NS对照组、TACE+罗勒胶囊大剂量组、TACE+罗勒胶囊中剂量组和TACE+罗勒胶囊小剂量组5组,除对照组外,其余4组大鼠均进行TACE术,术后分别给予不同剂量罗勒胶囊灌胃治疗。10d后处死大鼠,取出瘤块,免疫组化法检测瘤块中HIF-1α、MMP-2和MMP-9的表达情况,应用酶联免疫吸附试验(ELISA)法测定大鼠血清中AFP表达情况。结果:与TACE+生理盐水组比较罗勒胶囊大剂量组瘤质量明显降低(P<0.05),与非TACE对照组比较,TACE+NS组HIF-1α、MMP-2和MMP-9的表达均增高;罗勒胶囊治疗组HIF-1α、MMP-2和MMP-9表达较TACE+NS组表达显著降低,并呈现一定的剂量依赖性。TACE+NS组较非TACE组血清中AFP表达显著降低。罗勒胶囊大、中剂量治疗组较非TACE组血清中AFP显著降低,而与TACE+NS组比较,差异无统计学意义,P>0.05。肝癌组织中的HIF-1α与MMP-2的表达成正相关,P<0.01。结论:罗勒胶囊对大鼠移植性肝癌具有改善TACE术后乏氧的作用,并通过降低术后肿瘤乏氧微环境中HIF-1α和MMP-2及MMP-9的表达,而起到TACE术对肝移植瘤的抑制作用。更多还原

【Abstract】 OBJECTIVE:To observe the influence on expressions of HIF-1α,MMP-2,MMP-9 and AFP of serum in hypoxia environment after TACE of rat hepatoma with Basil capsule.METHODS:The rats were randomly assigned into five groups after the models were build:a group without TACE;TACE+NS group;TACE+mini-dose Basil capsule group;TACE+ middle-dose Basil capsule group;TACE+ large dose Basil capsule group.For the TACE group,the heal of TACE was simulated.For the TACE+ Basil capsule groups the different doses of Basil capsule were administered.Ten days later the rats were killed and the liver tumors were collected and dyed with HE.The expressions of HIF-1α,MMP-2 and MMP-9 in hepatoma focus were detected with immunohistochemistry.The levels of AFP were detected with ELISA.RESULTS:The tumor weight of large dose Basil capsule group deereased obvioushy than that in the TACE+NS group(P<0.05)compared with the group without TACE,the TACE+NS group had higher expressions of HIF-1α,MMP-2 and MMP-9,while different-dose Basil capsule goups had lower expressions than the TACE+NS group had.The TACE+NS group had lower expressions than the group without TACE had.The TACE+ middle-dose Basil capsule and TACE+ large-dose Basil capsule groups had lower the expressions than the group without TACE had,but the expressions were not different from those in the TACE +NS group(P>0.05).The expression of HIF-1α was positively correlated with that of MMP-2 in liver cancer tissues(P<0.01).CONCLUSIONS:Basil capsule can obviously improve hypoxia environment in rat hepatoma after TACE and down-regulate the expressions of HIF-1α,MMP-2 and MMP-9.Basil capsule has a dose dependent inhibitory action after TACE of rat hepatoma.更多还原