【摘要】 目的:研究SKP2和p16蛋白在肺癌组织中的表达,探讨它们之间的关系及其临床意义。方法:利用组织芯片和免疫组化技术检测SKP2和p16蛋白在89例非小细胞肺癌(NSCLC)、13例小细胞肺癌(SCLC)及10例肺良性病变组织中的表达。结果:NSCLC、SCLC及肺良性病变组织中SKP2和p16蛋白表达阳性率分别为(23.52±13.57)%、(44.72±15.97)%,(53.85±12.26)%、(28.25±5.68)%及(2.91±1.27)%、(91.13±6.57)%,三者之间差异有统计学意义,P值均为0.000。SKP2蛋白在肺癌组织中的表达水平与细胞分化程度、病理类型、TNM分期及淋巴转移密切相关,P值均为0.000。p16蛋白在肺癌组织中的表达水平与细胞分化程度,病理类型,TNM分期,淋巴转移密切相关,P值均为0.000。肺癌组织中SKP2和p16蛋白表达呈负相关,r=-0.660,P=0.000;SKP2蛋白表达率与肿瘤的恶性程度呈负相关,r=-0.286,P=0.000;p16蛋白表达率与肿瘤的恶性程度呈正相关,r=0.132,P=0.000。结论:SKP2蛋白表达增高与p16蛋白表达降低共同在肺癌的发生发展中起促进作用,且它们对肿瘤的恶性程度也有相反的影响,但它们之间相互作用的具体机制尚需进一步研究。更多还原

【Abstract】 OBJECTIVE:To explore the expressive characteristics of SKP2 and p16 proteins in lung carcinoma tissues and their affection to patients’prognosis. METHODS:The expressions of SKP2 and p16 proteins were detected in 89 NSCLC,13 SCLC,10 lung benign lesion tissues by tissue chip and immuno-histochemistry technology. RESULTS:The positive rates of SKP2 and p16 protein stain were (23.52±13.57)% and (44.72±15.97)%,(53.85±12.26)% and (28.25±5.68)%,(2.91±1.27)% and (91.13±6.57)% in NSCLC,SCLC and lung benign lesion tissues,respectiveely. There were significantly differences among them(P=0.000). The expressive level of SKP2 protein in lung carcinoma tissues was closely related to the cell differentiation,pathological type,TNM stage and lymph node metastasis (P=0.000). The expressive level of p16 protein in lung carcinoma tissues was closely related to the cell differentiation,pathological type,TNM stage,lymph node metastasis (P=0.000). The expression of SKP2 protein was negative correlation with p16 protein in NSCLC(r=-0.660,P=0.000). The expression rate of SKP2 protein in lung carcinoma was negative correlation with tumorous malignant degree(r=-0.286,P=0.000). The expression rate of p16 protein in NSCLC was positive correlated with tumorous malignant degree(r=0.132,P=0.000). CONCLUSION:The high expression of SKP2 protein and low expression of p16 protein concurrently play a facilitative role in the occurrence and development of lung carcinoma. Moreover,there i s a certain opposite role to lung tumorous malignant degree. Yet,the interactive mechanism between them needs further research.更多还原