【摘要】 目的:探讨COX-2和突变性p53蛋白在非小细胞肺癌(NSCLC)发生、发展中的作用机制及两者之间的相关性。方法:流式细胞仪检测80例NSCLC组织和20例癌旁正常肺组织中COX-2蛋白、突变型p53蛋白的表达情况。结果:NSCLC组织中COX-2蛋白较正常组织高表达,P<0.01。COX-2表达与有无淋巴结转移(P=0.002)和肿瘤大小(P=0.045)有关,与NSCLC患者性别、TNM分期、分化程度和组织学类型无关,P>0.05。NSCLC组织中p53蛋白较正常组织高表达,P<0.01。p53蛋白表达与有无淋巴结转移(P=0.01)有关;而与NSCLC患者性别、肿瘤的大小、TNM分期、肿瘤分化程度和组织学类型无关,P>0.05。NSCLC组织中p53和COX-2蛋白同时阳性表达率为47.82%(22/46),p53阴性COX-2蛋白阳性表达率为58.82%(20/34),两者表达无相关性,P=0.33。结论:COX-2的过表达及p53蛋白突变与NSCLC的发生发展有关,两者之间无相关性。更多还原

【Abstract】 OBJECTIVE:To investigate the expressions of COX-2 and p53 in human non-small cell lung cancer tissues and their correlation with biological behaviors of non-small cell lung cancer.METHODS:The expressions of COX-2 protein and mutation p53 protein were detected by flow cytometry in tissues of 80 cases of non-small cell lung cancer and 20 normal tissues adjacent to cancer.RESULTS:A high level of COX-2 protein was expressed in non-small cell lung cancer tissues compared with the normal tissues(P<0.01).The expression of COX-2 was significantly correlated with the lymph node metastasis(P=0.002) and tumor size(P=0.045),but not with their sexes and TNM stages,tumor grade,histologic type(P>0.05).A high level of p53 protein was expressed in non-small cell lung cancer tissues compared with the normal tissues,P<0.01.The expression of p53 was significantly correlated with the lymph node metastasis(P=0.01),but not with their sexes and TNM stages,tumor size tumor grade,and histologic type(P>0.05).The positive rate of p53 and COX-2 was 47.82%(22/46),and the positive rate of COX-2 with negative expression of p53 was 58.82%(20/34),P=0.33.CONCLUSION:COX-2 over expresson and p53 mutantion are correlated with the genesis and progress of non-small cell lung cancer,but there is no significant correlation between COX-2 protein and p53 protein.更多还原