【摘要】 目的:探讨曲古抑菌素A(TSA)在体外诱导急性早幼粒白血病HL-60细胞凋亡的机制。方法:采用MTT方法检测TSA对HL-60细胞增殖的影响,流式细胞术检测细胞周期和细胞凋亡,RT-PCR检测凋亡相关基因Bax、Caspase-9和Caspase-3的表达。结果:0.1μmol/L的TSA可明显抑制细胞增殖,P<0.01;0.05μmol/L的TSA可使细胞周期阻滞在G0/G1期(P<0.05),但0.1μmol/L的TSA才能引起细胞凋亡(P<0.01);经0.1μmol/L的TSA作用12 h后,Bax、Caspase-9和Caspase-3基因表达明显升高,P<0.01。结论:TSA诱导HL-60细胞凋亡的机制在于引起细胞周期阻滞,上调Bax、Caspase-9和Caspase-3的表达。更多还原

【Abstract】 OBJECTIVE: To explore the apoptosis mechanism of HL-60 cell induced by TSA.METHODS: The MTT method was used to test HL-60 cell growth.The cell cycle and apoptosis were tested by FCM.Semi-quantitative RT-PCR was used to detect the mRNA expressions of Bax,Caspase-9 and Caspase-3 in the cells treated by TSA.RESULTS: A decrease in cell growth was observed when the cells were treated by 0.1 μmol/L TSA,P<0.01.The cells were significantly accumulated at the G0/G1 phase under the treatment of 0.05 μmol/L TSA(P<0.05).However,the But cell apoptosis was only observed when the concentration of TSA was over 0.1 μmol/L(P<0.01).The expressions of Bax,Caspase-9 and Caspase-3 were up regulated by the exposure of HL-60 cells to 0.1 μmol/L TSA for 12 hours,P<0.01.CONCLUSION: The mechanism of HL-60 cell apoptosis induced by TSA is related to the up-regulation of Bax,Caspase-9 and Caspase-3.更多还原