【摘要】 目的观察大鼠肢体缺血再灌注(LIR)后骨骼肌细胞的凋亡情况及缺血预适应(IPC)对其影响。方法实验用雄性Wistar大鼠24只,随机分为对照(Control)组、缺血再灌注(IR)组和缺血预适应(IPC+IR)组,每组8只。分别测定血浆乳酸脱氢酶(LDH)、肌酸激酶(CK)、活性氧(ROS)、丙二醛(MDA)的含量;观察骨骼肌组织中谷胱甘肽过氧化物酶(GSH-PX)、还原型谷胱甘肽(GSH)、Ca2+、Na+-K+-ATP酶、Ca2+-Mg2+-ATP酶的含量变化及Caspase-3和骨骼肌凋亡指数(AI)。结果IPC+IR组较IR组,血浆LDH、CK、ROS、MDA及骨骼肌组织中Ca2+含量下降,骨骼肌组织中GSH-PX、GSH、Na+-K+-ATP酶、Ca2+-Mg2+-ATP酶水平升高,Caspase-3及骨骼肌AI下降。结论IPC可能通过减少自由基生成和提高自由基清除酶的活性、降低钙超载、抑制骨骼肌Caspase-3蛋白的表达,从而抑制大鼠LIR后骨骼肌细胞凋亡。更多还原

【Abstract】 Objective To investigate the apoptosis of skeletal muscle following limbs ischemia reperfusion(LIR) and the effects of ischemic preconditioning(IPC).Methods Using limb ischemia reperfusion injury model rats,the animals were randomly divided into control group,IR group,IPC+IR group.The contents of lactate dehydrogenase(LDH),creatine phosphate kinase(CK),reactive oxygen species(ROS),malondialehyde(MDA) in plasma and the values of glutathione peroxidase(GSH-PX),glutathione(GSH),Ca2+,Na+-K+-ATPase,Ca2+-Mg2+-ATPase in skeletal muscle were measured.The levels of Caspase-3 and the apoptosis index(AI) in skeletal muscle were detected.Results Compared with IR group,the values of LDH,CK,ROS,MDA in plasma were all decreased in IPC group.The levels of Ca2+ in skeletal muscle decreased in IPC group,but the levels of GSH-PX,GSH,Na+-K+-ATPase,Ca2+-Mg2+-ATPase increased.The expression of Caspase-3 and AI were lower in IPC group.Conclusion IPC inhibits the occurrence of apoptosis of skeletal muscle following the LIR injury in rats possibly by means of alleviating the damage of oxidative and the calcium overload,reducing the expression of the Caspase-3.更多还原