【摘要】 目的探索大鼠骨髓源性肝干细胞(BDHSC)脾内移植后的分化情况。方法利用肝细胞生长因子(HGF)体外诱导大鼠BDHSC,并植入60只成年SD大鼠脾内,两组(n=30)分别于术后7天和14天处死动物后取脾,RT-PCR及免疫组化检测移植细胞的AFP及白蛋白的表达。结果移植后7天,AFPmRNA、白蛋白mRNA和AFP的阳性表达率分别为73.3%、46.7%、56.7%;移植后14天,AFPmRNA阳性表达率(23.3%)显著下降(P<0.01),白蛋白mRNA阳性表达率(20%)显著下降(P<0.05),AFP阳性表达率(26.7%)亦显著下降(P<0.05)。结论体外诱导大鼠BDHSC脾内移植后,其原有的肝干细胞特性逐渐丧失更多还原

【Abstract】 Objective To explore the differentiation of rat bone marrow derived hepatic stem cell(BDHSC) after intrasplenical transplantation.Methods BDHSC was induced by hepatocytes growth factor in vitro,7 days and 14 days after intrasplenical transplantation,60 rats(two groups,n=30) were separately put to death for spleen,RT-PCR and immunohistochemistry were used to examine the expression of AFP and albumin in the BDHSC.Results 7 days after transplantation,the positive rate of AFP mRNA、albumin mRNA and AFP was 73.3%、46.7%、56.7%;14 days after transplantation,the positive rate of AFP mRNA(23.3%) decreased significantly(P<0.01),the positive rate of albumin mRNA(20%) decreased significantly(P<0.05),and the positive rate of AFP(26.7%) also decreased significantly(P<0.05).Conclusion BDHSCs in vitro might gradually lose their acquired characters of hepatocytes after intrasplenical transplantation.更多还原