【摘要】 采用固相pH梯度SDS聚丙烯酰胺双向凝胶电泳技术对杀雄剂SQ-1处理和未处理的小麦(Triticum aestivum)单核期、二核期花药总蛋白进行了分离,通过考马斯亮蓝G-250染色,获得了分辨率和重复性较好的双向电泳图谱。PDQuest2DE图像分析软件分析结果表明:(1)在等电点(pI)4～7之间可识别约500个以上较为清晰的蛋白质点,处理和对照各时期蛋白质在pI5～6,分子量20～40kD之间相对较集中。(2)检测到单核期差异点43个,二核期差异点45个,计算各差异点的分子量和pI。单核期43个差异点中,11个点在处理材料的单核期缺失而在对照中表达,7个在处理中表达而在对照中缺失;14个点表达量在处理中明显减弱,而另外11个明显增强。(3)二核期45个差异点中,有13个在处理中缺失而在对照中表达,7个在处理中表达而在对照中不表达;12个表达量在处理中明显减弱而另外13个明显增强。(4)经SQ-1处理后在单核期和二核期A-Z3(26.8/5.7)和C-Z5(26.8/5.7)点均缺失;点A-L10(26.8/5.9)在单核期处理的表达量下调,而到了二核期完全缺失C-Z6(26.9/5.9)点。通过双向电泳技术所获得的这些差异蛋白很可能直接或间接地参与了供试材料花药正常发育与败育发育的某些关键途径,而处理和对照互为等生理差异系,两者图谱中表现出的差异蛋白又很可能与SQ-1诱导小麦雄性不育有关。更多还原

【Abstract】 The anther proteins in uninucleate-stage and binucleate-stage from wheat(Triticum aestivum) male sterile line induced by chemical hybridizing agent SQ-1 were separated by two-dimensional electrophoresis with immobilized pH(4～7) gradients as the first dimension and SDS-PAGE as the second.Results showed that:(1) After Coomassie Blue G-250 staining and analysis by PDQuest 2DE software, over 500 protein spots were detected reproducibly, and these protein spots mainly focused on pI 5～6, molecular weight ranging 20.0 ～40.0 kD.(2) Forty three in uninucleate-stage and forty five in binucleate-stage differentially expressed protein spots were detected by PDQuest 2DE software, molecular weight and pI of these spots were calculated.In uninucleate-stage, 11 protein spots were absent in the protein map of treatment anther but present in that of check, 7 present in that of check but absent in that of treatment;14 down-regulated and 11 up-regulated significantly in that of treatment in comparison with that of check.(3) In binucleate-stage, 13 protein spots were absent in the protein map of treatment anther but present in that of check, 7 present in that of check but absent in that of treatmen;12 down-regulated and 13 up-regulated significantly in that of treatment.(4) The protein spots A-Z3(26.8/5.7) in uninucleate-stage and C-Z5(26.8/5.7) in binucleate-stage were absent in the protein map of treatment anther;The spot A-L10(26.8/5.9) of treatment anther in uninucleate-stage was down-regulated but spot C-Z6(26.9/5.9) absent in binucleate-stage.These differential proteins may participate in pollen normal development and abortion process directly or indirectly, moreover, treatment and check are iso-physiological different line each other, it can be deduced that the differentially expressed protein spots may relate to male sterility induced by CHA-SQ-1.更多还原