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一个斯卑尔脱小麦早熟突变体抽穗期的遗传分析与分子标记初步定位

Genetic Analysis and Preliminary Molecular Mapping of the Heading Date in an Early-maturity Spelt Wheat Mutant

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【作者】 王军丁玉华彭惠茹王晓娜谢允慧刘志勇倪中福孙其信

【Author】 WANG Jun,DING Yu-hua,PENG Hui-ru,WANG Xiao-na,XIE Yun-hui,LIU Zhi-yong,NI Zhong-fu,SUN Qi-xin(State Key Laboratory for Agrobiotechnology,China Agricultural University/Key Laboratory of Crop Heterosis and Utilization,Ministry of Education/Key Laboratory of Crop Genomics and Genetic Improvement,Ministry of Agriculture /Beijing Key Laboratory of Crop Genetic Improvement,Beijing 100193,China)

【机构】 中国农业大学农业生物技术国家重点实验室,教育部杂种优势研究与利用重点实验室,农业部作物基因组与遗传改良重点实验室,北京市作物遗传改良重点实验室

【摘要】 研究以斯卑尔脱小麦(Triticum spelta)Hubel及其早熟诱变系2463为材料,采用主-多基因混合模型多世代联合分析发现,抽穗期性状由两个主基因控制,其遗传力为44%~81%。温室春化和光周期处理实验结果表明,经过春化处理后,与Hubel相比,其早熟诱变系在长日照条件下的抽穗期提前10d左右,表现为显著差异。利用F2分离群体进行的分子标记定位结果表明,在2D染色体的短臂上存在一个主效QTL,与标记Xwmc112紧密连锁,能解释表型变异的16%,与前人定位的光周期相关QTL/基因(Ppd1)位于同一区域。

【Abstract】 The inheritance of an early-maturity spelt wheat(Triticum spelta) Hubel and mutant line 2463 were analyzed using major-polygene mixed inheritance model.The results indicated that heading date was controlled by two major genes,and their heritability was 44%~81%.Further analysis revealed that,after vernalization,the heading date of Hubel was about 10 days later than that of mutant line 2463.Moreover,using F2 population derived from cross combination 2463/Hubel,one QTL controlling early heading date,which tightly linked to SSR marker Xwmc112,was mapped on the short arm of chromosome 2D,and this QTL could contribute for 16% of the observed phenotypic variation of heading date and located on the same chromosome region to QTL photoperiod sensitive gene(Ppd1) mapped in previous study.

【基金】 国家重点基础研究发展规划(973)项目(No.2007CB109000和2006CB101706)资助
  • 【文献出处】 农业生物技术学报 ,Journal of Agricultural Biotechnology , 编辑部邮箱 ,2009年04期
  • 【分类号】S512.1
  • 【被引频次】8
  • 【下载频次】208
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