【摘要】 选取长期堆放红霉素药渣的土壤,经驯化富集后筛选到1株能高效降解红霉素的菌株,根据表型特征、生理生化特性及18S rDNA序列同源性分析,鉴定为红酵母属的粘性红圆酵母(Rhodotorula mucilaginosa)。该菌的18S rDNA序列片段长度为1562bp,在GenBank登录号为EU294522。该菌可以利用红霉素作为碳源生长,适用碳源与氮源分别为蔗糖和NH4Cl。该菌降解红霉素的最适温度为30℃,最适pH5.0～5.5,最适接菌量6%,降解率与通气量成正相关。在最适反应条件下培养48h,该菌株对红霉素的降解率可达100%。更多还原

【Abstract】 An erythromycin-degrading strain was isolated from soil contaminated by erythromycin. Based on analysis of its morphology, 18S rDNA, physiological and biochemical characteristics, the strain was identified to be Rhodotorula mucilaginosa. Its sequence of 18S rDNA was 1 562 bp and the accession number was EU294522 in GenBank. The strain could grow using erythromycin as the carbon source, optimal carbon and nitrogen sources were sucrose and NH4Cl, respectively. The optimal temperature, pH and inoculation for erythromycin degradation were 30 ℃, 5~5.5, and 6% , respectively, and he degradation rate was positively related to aeration, and could be 100% after the strain was cultured for 48 h.更多还原