【摘要】 利用可培养和变性梯度凝胶电泳(DGGE)方法,对经不同浓度TiO2处理黄瓜(Cucumis sativus)叶围细菌群落结构的变化进行了分析。研究发现,TiO2处理后黄瓜叶围可培养细菌数量较对照显著降低。随着TiO2浓度从0.02 mg/mL提高至20mg/mL,黄瓜叶围可培养细菌数量从1.8×107 cfu/g降低至3.1×106 cfu/g。DGGE指纹图谱分析表明,当TiO2的喷雾浓度超过0.02 mg/mL时,黄瓜叶围细菌的多样性显著降低。对DGGE条带克隆测序结果显示,在黄瓜叶围至少存在7种不同属的细菌,其中只有一种叶围细菌不受TiO2喷雾浓度影响。更多还原

【Abstract】 The impact of nanometer titanium dioxide(nano-TiO2) to the phyllosphere bacterial community were assessed using cul-turable-depend and PCR-DGGE methods.Results showed that quantities of the culturable phyllosphere bacteria were reduced from 1.8×107 to 3.1×106 cfu/g with the increasing concentrations of the nano-TiO2(from 0.002 to 20 mg/mL).The diversity of the phyllo-sphere bacteria was also analyzed by PCR-DGGE.When the concentrations of the nano-TiO2 were higher than 0.02 mg/mL,the DGGE bands was significantly lower than that of the control.Sequencing results of the bands from the DGGE gel showed that there were at least 7 bacterial genera on the cucumber phyllosphere.But only one phyllosphere uncultured bacterium was not influenced by the concentration of the nano-TiO2.更多还原