【摘要】 利用Real-time PCR技术对中华蜜蜂(Apis cerana cerana)两个化学通讯相关蛋白:气味结合蛋白Ac-ASP2和化学感受蛋白Ac-ASP3基因的时空转录谱进行了研究。结果表明,Ac-ASP2是一个特异表达于中蜂触角的基因,在幼虫及蛹中均不表达;利用2-ΔΔCt的相对定量方法,发现Ac-ASP2在成虫触角中具有几个丰度较高的日龄,即1、9、15、27和30日龄,这几个高表达日龄均较其它时期高出近10倍以上;利用标准曲线的绝对定量方法,Ac-ASP3在其不同器官中的原始拷贝数为:翅>腹>胸>足>触角>头,其中翅、腹和胸部模板拷贝数较高(均达到106数量级),而在足、触角以及头中的拷贝数相对较低(均为105数量级),表明Ac-ASP3与中蜂翅与腹部的化学感受行为有密切的关系。更多还原

【Abstract】 The spatio-temporal expressed profiles of two kinds of chemical communication related to genes of Apis cerana cerana proteins,odorant-binding protein of Ac-ASP2 and chemosensory protein of Ac-ASP3,were identified by Real-time PCR.Using the method of 2-ΔΔCt,the results with the method of 2-ΔΔCt showed that Ac-ASP2 was a gene coding antenna specific protein,which did not express in larva and pupa,but had discontinuous high abundance periods: 1,9,15,27 and 30 d,the expressing abundance at such pe-riods was tenfold higher than that at other periods.From the distribution of Ac-ASP3 mRNA in different issues,the transcript amounts seemed to be higher in wings,abdomen and thorax(about 106 order of magnitude),and lower in legs,antennae and head(about 105 order of magnitude),the original copy number was: wings >abdomen >thorax >legs >antennae >head.The results above showed that Ac-ASP3 had intimate relation with chemosensory behavior of wings and abdomen in Apis cerana cerana.更多还原