【摘要】 目的:研究纳米二氧化钛(TiO2)对雄性小鼠的在体作用,主要观察其对雄性小鼠生殖系统的影响。方法:45只6周龄的雄性ICR小鼠随机均分3组,实验组隔日腹腔注射纳米TiO2(200mg/kg或500mg/kg),对照组注射等体积生理盐水,共给药5次。停药1周后,测量心脏、肝、肾、脾、睾丸和附睾的脏器系数,血清生化指标、睾酮和雌二醇水平;显微镜下观察附睾精子数量、活率、畸形率和睾丸内精子计数;主要脏器作病理切片和HE染色,TUNEL法检测睾丸生殖细胞凋亡。结果:与对照组相比,给药200mg/kg纳米TiO2组上述指标均无明显改变(P>0.05);给药500mg/kg组小鼠的心、肝和肾质量系数显著降低(P<0.05);丙氨酸氨基转移酶(ALT)、丙氨酸氨基转移酶/天门冬氨酸氨基转移酶(ALT/AST)、尿素氮(BUN)均显著升高(P均<0.05);附睾精子数、精子活率以及睾丸内精子计数均显著降低,精子畸形率增高,睾丸生殖细胞凋亡明显增多(P均<0.05)。肝、肾、脾、睾丸和附睾的病理切片观察未见明显改变。结论:小剂量的纳米TiO2对雄性小鼠无明显影响,较大剂量的纳米TiO2对雄性小鼠肝、肾功能有轻度影响,对小鼠精子生成和精子功能有明显影响,并诱导睾丸生殖细胞凋亡。更多还原

【Abstract】 Objective:To investigate the in vivo effects of nanosized titanium dioxide (TiO2) on the main organs, particularly the reproductive system, of male mice. Methods: Forty-five male ICR mice aged 6 weeks were equally and randomly divided into 2 experimental groups and a control group, intraperitoneally injected with 200 and 500 mg/kg nanosized TiO2 and equal volume of saline, respectively, every other day for 5 times. One week after drug cessation, we obtained the coefficients of the main organs, serum biochemical parameters and the levels of gonadal hormones (T and E2), analyzed the pathological changes of the main organs by HE staining, observed sperm count, motility and abnormality under the microscope, and detected germ cell apoptosis by TUNEL. Results: Compared with the control, the low-dose (200 mg/kg) group showed no significant changes in the above parameters, while the high-dose (500 mg/kg) group exhibited a decrease in the coefficients of the liver, heart and kidneys, and a significant increase in such serum biochemical parameters as ALT, ALT/AST and BUN (P < 0.05). The high-dose group also showed significantly reduced sperm density and motility, increased sperm abnormality and germ cell apoptosis (P < 0.05), but no obvious pathological changes in the liver, kidney spleen, testis and epididymis. Conclusion: Low-dose nanosized TiO2 has no obvious effect on male mice, but high-dose may significantly reduce their sperm count and function and induce germ cell apoptosis, although its damage on the liver and kidney function is slight.更多还原