【摘要】 目的:比较不同周期妊娠率的宫腔内人工授精(IUI)所用的精子中,精子蛋白ERp57表达量的差异,探讨其能否作为筛选合格供精者精液的一项指标。方法:将42例用于IUI的精液标本,按IUI周期妊娠率分为A组(16例,IUI周期妊娠率为0)、B组(13例,IUI周期妊娠率为10%～20%)和C组(13例,IUI周期妊娠率大于20%),应用Western印迹检测精子蛋白ERp57的表达情况,其表达量用ERp57蛋白与内参β-微管蛋白的平均吸光度的比值表示。结果:A、B、C组精子蛋白ERp57的表达量分别为:0.95±0.24、1.33±0.43和1.33±0.39,ERp57蛋白在A组中的表达量明显低于B组与C组(P<0.05),而B组与C组之间则无明显差异(P>0.05)。结论:ERp57蛋白在A组(IUI周期妊娠率为0)精子中的低表达,可以作为筛选不合格供精者及精子库中低IUI周期妊娠率的精液标本原因分析的一项参考指标。更多还原

【Abstract】 Objective:To investigate the association between the pregnancy rate of intrauterine insemination (IUI) and the ERp57 expression level in donor sperm.Methods:Forty-two sperm samples were divided into 3 groups according to the pregnancy rate:Group A (n = 16,pregnancy rate = 0),B (n = 13,pregnancy rate 10%-20%) and C (n = 13,pregnancy rate ≥ 20%). The optical density (OD) was normalized to the β-tubulin band for the evaluation of the ERp57 level.Results:OD (ERp57/Tubulin) was 0.95 ± 0.24 in Group A,1.33 ± 0.43 in Group B and 1.33 ± 0.39 in Group C. The ERp57 expression level was significantly lower in Group A than in B and C (P < 0.05),with no significant differences between the latter two groups.Conclusion:The ERp57 expression level in donor sperm could be used as an index to predict the pregnancy rate of IUI and to avoid IUI failure by removing low-level donor sperm.更多还原