【摘要】 对虾夷马粪海胆(Strongylocentrotus interm edius)患"黑嘴病"及生殖后体腔液中5种生理生化指标进行了检测,结果表明病理情况下的虾夷马粪海胆过氧化氢含量和丙二醛含量明显高于对照组,而过氧化氢酶活性、超氧化物歧化酶活性和总抗氧化能力明显低于对照组,其中,过氧化氢含量、总抗氧化能力和超氧化物歧化酶活性差异显著(P<0.05),丙二醛含量、过氧化氢酶活性差异极显著(P<0.01)。生殖后除过氧化氢含量外其他4种生理生化指标均降低,其中总抗氧化能力和丙二醛含量差异显著(P<0.05),过氧化氢酶和超氧化物歧化酶活性差异极显著(P<0.01)。研究表明以海胆体腔液中丙二醛含量和过氧化氢酶活性作为海胆黑嘴病发生的监测指标是可行的。更多还原

【Abstract】 Five physiological and biochemical parameters were studied in sea urchins（Strongylocentrotus inter-medius） suffering from black mouth disease and after breeding.The results showed that under pathological status,the H₂O₂ content and malondialdehyde（MDA） content were significantly higher than those of control sea urchins,while catalase（CAT） activity,superoxide dismutase（SOD） activity and total antioxidant capacity（T-AOC） were notablely lower than those in control ones.Among these parameters,the H₂O₂ content,T-AOC and SOD activity were significantly different（P<0.05） between control and sick groups,while the difference of MDA content and CAT activity were extremely significant（P<0.01） between them.After breeding,the H₂O₂ content remained while the other four types of immune parameters decreased,among which the T-AOC and MDA content were significantly different（P<0.05）,while the difference of CAT activity and SOD activity between after and before breeding were extremely significant（P<0.01）.The results suggest that the content of MDA and CAT of coelomic liquid can be used as monitoring indicators of black mouth disease in sea urchins.更多还原