【摘要】 目的:探讨胎盘绒毛组织孕激素受体(progesterone receptor,PR)表达变化与分娩发动的关系。方法:采用免疫组化和Westernblot印迹杂交法检测正常足月剖宫产产妇胎盘绒毛组织(临产组15例,未临产组15例)孕激素受体表达变化情况。结果:免疫组化结果显示15例未临产组均存在核PR染色阳性,15例临产组出现弱阳性结果。Westernblot结果显示,临产组PR-A表达量较未临产组下调,同时临产组PR-B表达量较未临产组也下调,两者均存在显著性差异,具有统计学意义(P<0.01);临产组PR-A/PR-B较未临产组高,存在统计学意义(P<0.05)。结论:PR-A/PR-B比值升高可能是参与分娩发动的机制之一。更多还原

【Abstract】 Objective:To disscuss the relationship between progesterone receptor(PR)expression in human placenta chorionic tissues and labor onset .Methods:PR protein levels and cellular localization were measured by immunohistochemistry and Western blot,respectively,in term pregnancy placenta tissue .Two clinical groups were selected:woman in labor(n = 15)and not in labor(n = 15).Results:PR immunostaining in placenta tissues of women not in labor was positive,and slightly positive in placenta tissues of women in labor.PR-A and PR-B levels decreased in labor group(P < 0.01) by Western blot.The PR-A to PR-B protein ratio also increased in labor group(P < 0.05).Conclusion:An increased PR-A to PR-B ratio may participate in the process of labor onset.更多还原