【摘要】 目的:探讨高糖对大鼠肾小球系膜细胞TGF-β1表达的影响及Resveratrol的干预作用。方法:体外培养大鼠肾小球系膜细胞。①分为正常对照组(NC组,葡萄糖浓度5.6mmol/L)、高糖组(HG组,葡萄糖浓度30mmol/L),分别观察24、48、72h;②分为NC组、HG组、Resveratrol组(Res组,葡萄糖浓度5.6mmol/L+Resveratrol20μmol/L)和高糖+Resveratrol组(HG+Res组,葡萄糖浓度30mmol/L+Resveratrol浓度分别为5、10、15、20μmol/L),各组细胞分别培养48h。用半定量RT-PCR和Western blotting法检测细胞内TGF-β1mRNA和蛋白表达变化。结果:①与NC组相比,HG刺激后大鼠系膜细胞TGF-β1mRNA和蛋白表达明显增加,差异均有显著性,P值均<0.01;②与HG组相比,HG+Resveratrol干预后,大鼠系膜细胞TGF-β1mRNA和蛋白表达呈浓度依赖性减少,差异均有显著性(P值分别为<0.05,<0.01)。Res组和NC组之间TGF-β1mRNA和蛋白表达的差异无显著性(P>0.05)。结论:高糖能上调大鼠肾小球系膜细胞TGF-β1mRNA和蛋白表达,Resveratrol可能通过抑制高糖引起的系膜细胞TGF-β1高表达而在糖尿病肾病中起治疗作用。更多还原

【Abstract】 Objective:To investigate the effect of Resveratrol(Res)on the expression of high glucose-activated transforming growth factor-β1(TGF-β1) in rat renal mesangial cells.Methods:①Cultured rat renal mesangial cells were exposed to D-glucose at 30 mmol /L for periods of 24,48,72 h,respectively.Normal glucose(5.6 mmol /L) served as control.②Cultured rat renal mesangial cells were divided into four groups:normal glucose(5.6 mmol /L),high glucose(30 mmol /L),normal glucose plus Resveratrol(20 μmol /L),high glucose plus Resveratrol at 5,10,15,20 μmol /L,respectively for periods of 48 h.The mRNA levels of TGF-β1 were measured by RT-PCR and the protein levels of TGF-β1 were detected by Western Blotting.Results:As compared to normal cells,exposure of rat renal mesangial cells to D-glucose at concentrations of 30 nmol /L caused a time-dependent increase in the mRNA and protein levels of TGF-β1 and reached the highest level at approximately 48 h.The addition of Resveratrol significantly attenuated high glucose-enhanced mRNA and protein levels of TGFβ1(P < 0.05 or P < 0.01) in a dose-dependent manner.No notable difference at the mRNA and protein levels of TGF-β1 was found between Res and NC group(P > 0.05).Conclusion:High glucose contributes to an increasing of TGF-β1 expression at both the mRNA and protein levels in rat renal mesangial cells.Resveratrol can significantly reverse high glucose up-regulated expression of TGF-β1 in the rat renal mesangial cells and may play a role in the treatment of diabetic nephropathy.更多还原