【摘要】 目的:研究经脾脏注射未成熟树突状细胞(iDC)对大鼠小肠移植免疫耐受的诱导。方法:健康成年F344大鼠为供体,SD大鼠为受体。随机分为4组(n=10),A组(阴性对照组):移植前7天,受体经阴茎背静脉注射生理盐水1ml;B组(阴性对照组):移植前7天,受体经脾脏注射生理盐水1ml;C组(iDC阴茎背静脉注射组):移植前7天,受体经阴茎背静脉注射供体的iDC,细胞数为2×106个;D组(iDC脾脏注射组):移植前7天,受体经脾脏注射供体的iDC,细胞数为2×106个。4组1周后行大鼠异位节段性小肠移植术。观察各组受体存活情况,移植小肠采用病理学检查及细胞凋亡检测。结果:D组大鼠移植小肠存活时间为(17.50±1.05)天,较A组(7.17±1.17)天、B组(7.33±1.51)天、C组(12.83±2.79)天显著延长;D组移植小肠炎性细胞浸润、黏膜组织结构破坏程度和黏膜细胞的凋亡数目明显低于A、B、C组。结论:经脾脏注射供体来源的iDC,可在一定程度上诱导受体产生免疫耐受,显著延长受体大鼠小肠移植术后的存活时间。更多还原

【Abstract】 Objective:To investigate the effects of intra-spleen injection with immature dendritic cells on donor-specific tolerance in rats receiving intestinal transplantation. Methods:DCs were collected from bone marrow progenitor cells of rats cultured with GM-CSF in vitro. The DCs were harvested after six-day culture and the expression of co-stimulation molecular and MHC-Ⅱwas detected. Severn days before transplantation of small intestinal from F344 donors, donor-derived DCs(2 × 106) were injected into either the peripheral vein(group C) or the spleen(group D). Normal saline was injected through either the peripheral vein(group A) or the spleen(group B) before transplantation. Small intestinal transplantation was performed and the survival rate of recipients was observed. Histological examination was done and apoptosis death of infiltrating cells in allografts was analyzed. Results:The survival of recipients in group D was (17.50 ± 1.05)days, which was significantly longer than that in group A [(7.17 ± 1.17)days],B [(7.33 ± 1.51)days] and C [(12.83 ± 2.79)days] respectively(P < 0.05). The degree of the inflammatory infiltrated cells and intestinal mucosa structural injury of mucosa in group D were alleviated significantly compared with that in group A,B and C. Conclusion:The intra-spleen injection of modified immature dendritic cells can induce immune tolerance and prolong the survival of rat small bowel allografts.更多还原