【摘要】 测定了疣尾蜥虎(Hemidactylus frenatus)的线粒体基因组全序列.序列全长为16 891 bp,包括13个蛋白质编码基因、2个rRNA基因和22个tRNA基因.除大部分基因由重链编码外,仅1个蛋白编码基因和8个tR-NA基因由轻链编码.碱基组成的偏好与其他脊椎动物线粒体DNA接近.没有发现长的基因间隔区,说明该基因组的结构十分紧凑.基因组的组成与典型脊椎动物的相近,即没有发现重排、基因或控制区的重复等在其它有鳞类动物中出现过的异常特征.除单性生殖的壁虎外,现有的壁虎类线粒体基因组在基因含量和顺序上是一致的.作为蜥虎属线粒体基因组全序列的惟一代表,该序列有望在有鳞类系统发生的推断上发挥一定的作用.更多还原

【Abstract】 We determined the complete mitochondrial sequence of the common house gecko,Hemidactylus frenatus,by using the polymerase chain reactions(PCRs).The entire mtDNA sequence is 16891bp in length and contains 13 protein-coding genes,2 rRNA genes,22 tRNAs genes and the noncoding control region.Most genes are encoded by the H strand,except for one protein-coding and eight tRNA genes.The base composition is skewed similar to other vertebrate mtDNAs.No long intergenic spacers are found indicating a compact composition of this genome.In gene organization,the mitochondrial genome of H.frenatus is identical to that of typical vertebrate,that is to say three main atypical features(rearrangements,duplication of genes and of the control region) found in some squamates are not found in this genome.Except the parthenogenetic geckos,known geckos have consistent gene contents and orders.The new complete sequence can be useful to improve the future phylogeny by broadening the coverage of squamate diversity.更多还原