【摘要】 根据禾谷镰孢菌(Fusarium graminearum)测序菌株NRRL31084(PH-1)的微管相关蛋白基因(map)核苷酸序列设计4对引物,采用PCR方法测定了禾谷镰孢菌对多菌灵(MBC)不同敏感性表型的6个中国菌株的map全序列。DNA序列比对表明,中国的3个敏感菌株和3个抗药菌株的map核苷酸序列相似性没有差异,表明多菌灵抗药性与微管相关蛋白无关。该基因全长1 793 bp,含有6个内含子,编码199个氨基酸;与NRRL31084的map核苷酸序列相似性为99.39%,存在10个差异核苷酸,与其所编码的氨基酸序列相似性为100%。更多还原

【Abstract】 The full-length nucleotide sequence of microtubule-associated proteins gene(map)from each of 6 Fusarium graminearum strains from China which had different carbendazim(MBC)sensitivity phenotypes were cloned using PCR with 4 primer pairs designed in accordance with nucleotide sequence of map from the genome-sequenced isolate,NRRL31084(PH-1).The DNA sequence comparison showed that there was no difference in the nucleotide sequence of map among 3 sensitive and 3 resistant strains from China.This result demonstrated that there was no relationship between MBC-resistance and map gene.The full-length of the gene spans 1 793 bp,including 6 introns,encoding 199 amino acids.With 99.39% homology,there were 10 nucleotide differences in map gene between PH-1 isolate and the 6 strains from China.The homology of the deduced amino acid sequence of the gene was 100% between the 6 strains and PH-1 isolate.更多还原