【摘要】 以OECD标准的基质染毒法测定了离子液体溴化1-辛基-3-甲基咪唑([C8mim]Br)对蚯蚓(Eisenia foetida)的急性毒性效应和亚慢性毒性条件下蚯蚓体内CAT、SOD、GST的活性和GSH、MDA含量的变化,以期初步分析[C8mim]Br对蚯蚓抗氧化系统的作用及其毒性作用的可能机理。结果表明,[C8mim]Br对蚯蚓的7d-LD50和14d-LD50分别为206.8mg.kg-1和159.4mg.kg-1。亚慢性暴露42d后蚯蚓体内CAT的活性受到显著抑制;SOD的活性在低浓度(1～5mg.kg-1)受到抑制,高浓度(20～40mg.kg-1)被激活;在高浓度处理组(20～40mg.kg-1)GST的活性显著高于对照。10～40mg.kg-1浓度的[C8mim]Br处理组GSH的含量显著升高,各处理组MDA含量与对照相比没有差异。推测[C8mim]Br可能通过肠道吸收进入蚯蚓体内,并诱导了蚯蚓体内抗氧化系统的反馈效应。更多还原

【Abstract】 This paper present the results of the acute toxicity determination of 1 methyl-3-octylimidazolium bromide([C8mim]Br)ionic liquid on earthworm Eisenia foetida and responses of the earthworm antioxidant system to the subchronic toxicity of this kind of ionic liquid,in which the activity of CAT,SOD,GST and the content of GSH and MDA were measured to evaluate the possible mechanism of [C8mim]Br to E.foetida.The results showed that:the 7 d and 14 d LD50 of ionic liquids to E.foetida were 206.8 mg.kg-1 and 159.4 mg.kg-1,respectively.And after 42 days of subchronic exposure to [C8mim]Br,the CAT activity of E.foetida were inhibited significantly,the SOD activity were also in-hibited at low concentrations(1～5 mg.kg-1)of [C8mim]Br,but were stimulated at high concentrations of 20～40 mg.kg-1.As for the activity of GST,it was significantly higher than the control at 20～40 mg.kg-1 of [C8mim]Br.The content of GSH increased than the control while the MDA content had no changes after exposure.According to the above results,we assume that [C8mim]Br might be absorbed by the earthworm gut or via the derma of earthworm,and then they disturb the metabolic reaction of earthworm and induce the feedback reaction of earthworm antioxidant system.更多还原