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梨形环棱螺保护酶分子标志物在大运河污染评价中的应用

Application of Protective Enzyme in Bellamya purificata as Molecular Biomarker in Pollution Assessment of the Grand Canal

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【作者】 刘缠民冯照军李宗芸

【Author】 LIU Chan-min1,2,FENG Zhao-jun1,LI Zong-yun1(1.School of Life Sciences,Xuzhou Normal University,Xuzhou 221116,China;2.Jiangsu Hi-Tech Key Laboratory for Eco-Agricultural Biotechnology Around Hongze Lake,Huai’an 223300,China)

【机构】 徐州师范大学生命科学学院江苏省环洪泽湖生态农业生物技术重点实验室

【摘要】 运用室内模拟法、样点笼内放养法和直接采样法研究京杭大运河不同污染程度环境对梨形环棱螺内脏团中超氧化物歧化酶(SOD)、过氧化物酶(POD)和过氧化氢酶(CAT)活性的影响。结果表明:梨形环棱螺组织抗氧化保护酶系统的SOD、POD、CAT活性是指示污染的敏感指标,其监测结果与水质化学评价结果基本一致。CAT活性在临界范围内随着污染程度加深,抑制作用加强。POD活性在污染环境中被激活,但各时间段的增幅不尽相同。SOD活性变化不稳定,较适合作为短时间内的监测指标。比较而言,这3种测定保护酶分子标志物活性的试验方法以样点笼内放养法最优,直接采样法最不可取。

【Abstract】 Effects of pollution of the Grand Canal on activities of the superoxide dismatase(SOD),peroxidase(POD)and catalase(CAT)in the innards of Bellamya purificata in the Grand Canal,by means of indoor simulation,cage raising in sampled plots and direct sampling.Results show that activities of SOD,POD and CAT,components of the antioxidant defense system,in Bellamya purificata,are sensitive indicators of exposure to pollution.The monitoring findings tallied with the data of water chemical assessment.Within the range below the threshold,CAT activity decreased with the intensifying of pollution,while POD was activated,but amplitude of the rise varied from time to time.SOD fluctuated in activity,and is fit to serve as a short-term indicator.By comparing the three methods in determining effect of pollution on activities of SOD,POD and CAT in Bellamya purificata,the method of cage-raising is the optimal one,and the sampling method is not applicable.

【基金】 江苏省高校自然科学基础研究项目(07KJD180210);江苏省环洪泽湖生态农业生物技术重点实验室开放基金(HZHL0811)
  • 【文献出处】 生态与农村环境学报 ,Journal of Ecology and Rural Environment , 编辑部邮箱 ,2009年02期
  • 【分类号】X824
  • 【被引频次】6
  • 【下载频次】142
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