【摘要】 目的:观察重组人表皮生长因子(rhEGF)对皮肤创面愈合的作用。方法:制作大鼠背部创伤模型,采用自身平行对照,将34只大鼠背部的68个创面分成rhEGF治疗组与盐水对照组,观察大体形态和组织学改变、创面愈合时间和愈合率,测定伤后不同时间创面羟脯氨酸(OHP)含量和Ⅰ型/Ⅲ型胶原比例,进行细胞DNA周期分析。结果:经rhEGF治疗的创面愈合速度较盐水对照明显加快,2组平均愈合时间为(1 7.2±1.3)d和(20.5±1.6)d(P<0.01);外用rhEGF使创面肉芽组织生成增多,再上皮化明显,显著增加创面中OHP含量,降低Ⅰ型/Ⅲ型胶原比例,加速细胞DNA复制。结论:外用rhEGF可缩短创面愈合时间,增加肉芽组织及OHP含量,降低Ⅰ型/Ⅲ型胶原比例,加速细胞DNA复制,明显促进皮肤创面的修复。更多还原

【Abstract】 Objective To observe the effects of recombinant human epidermal growth factor(rhEGF) on skin wound healing.Methods The dorsal trauma model of rats was used.A total of 68 dorsal wounds in 34 rats were creatd and divided into the rhEGF group and the isotonic saline group according to self-control.The process of wound healing was observed and a mean healing time was calculated.Furthermore,the dynamic analysis was performed at different times on wound OHP contents,ratio of collagenⅠ/Ⅲand cell DNA cycle.Results The wound healing was accelerated obviously in all wounds treated with rhEGF.The mean time of wound healing of the two groups was(17.2±1.3)days and (20.5±1.6)days respectively(P<0.01 ).In the rhEGF group,the new granulation tissue was more and the reepithelialization was faster than that of the saline group,External rhEGF increased OHP content,reduced collagenⅠ/Ⅲratio and acelerated cell DNA replication.Conclusion External rhEGF can shorten wound healing time,increase granulation tissue and OHP contents,reduce collagen l/lll ratio and accelerate cell DNA replication,thus obviously promoting skin wound repair.更多还原