【摘要】 目的:探讨外用中药复方紫归膏(Compound-ZiGui Cream,CZGC)促进大鼠皮肤创面愈合的作用及机制。方法:取SD大鼠125只,建立大鼠创伤模型。实验分成A、B、C、D、E共5组,每组25只。A组为模型组,B组为京万红对照组,C、D、E组分别为复方紫归膏高、中、低药物浓度剂量组。分别于伤后3、7、10、14天时间点观测创面愈合率、HE常规染色显微镜下新生肉芽组织厚度及其形态学改变情况,最后观测创面平均愈合时间等。结果:B、C、D、E组第7、10、14天创面愈合率均优于A组(P<0.01),C、D组第7、10天创面愈合率优于B组(P<0.05);B组第7、14天创面愈合率较E组优(P<0.01);B、C、D、E创面愈合时间均优于A组(P<0.01);C、D组优于B组(P<0.01);B组和E组间创面愈合时间无差异(P>0.05)。伤后第7、10天,B、C、D组肉芽组织厚度均较A组厚(P<0.01),E组第7、14天较A组厚(P<0.05),B组较E组厚(P<0.01)。组织病理图像分析,伤后第7天,B、C、D、E组新生毛细血管数、毛细血管总面积、面密度、数密度和周密度较A组均有明显提高(P<0.05或0.01),且C、D组作用优于B组(P<0.05或0.01)。B组优于E组(P<0.05)。结论:外用中药复方紫归膏能促进皮肤创面愈合,其可能机制是促进新生毛细血管的增殖,促进肉芽组织生长。更多还原

【Abstract】 Objective To investigate the effect and mechanism of compound preparation of TCM Zigui Cream ( CZGC)on rats skin wound. Methods Full thickness excisional wound on the back of rats were used as wound models.The 125 rats were divided randomly into 5 groups and treated with FanShiLing Cream (A),JingWangHong Cream(B),a large (C),middle (D),and small (E)doses of CZGC respectively.The therapeutic effects were evaluated by measuring the wound healing rate,the thickness of granulation tissue on the 3rd,7th,10th,14th days after wound,calculating the Total Area and Area density,Number and Number density,Perimeter density of capillary tubes of granulation tissue on the 7th by thepathology tectology (HE) ,and taking down the Average healing time at last. Results on the 7th,10th,14th after wound the wound healing rate,the thickness of granulation tissueof group C、D、E were increasing more obviously than those of groups A (P<0.01). Histopathology Image analysis showed on the 7th,the Total Area and Area density、 Number and Number density、Perimeter density of capillary tubes of granulation tissue of group C、D、E were obviously improved as compared with groups A (P<0.05 or P<0. 01) ,and The effect of group C、D were superior to the group B ( P<0.01 or P<0. 01). group C、D、E decurtate the Average healing time obviously ( P<0.01). Conclusion CZGC can accelerate healing of wound obviously.The mechanisms might be related to promoting the proliferation of capillary tubes,and facilitating the growth of granulation tissue.更多还原