【摘要】 目的:通过建立兔耳腹侧增生性瘢痕的模型,探讨积雪草甙(Asiaticoside,Ad)对TGF-β1mRNA表达的影响。方法:新西兰大白兔20只(喂养过程中死亡4只),在兔耳腹侧制备直径为1cm、深达软骨膜的圆形瘢痕93个,随机分为4组:空白对照组、得宝松组、低浓度积雪草甙组(25mg/ml)、高浓度积雪草甙组(50mg/ml),分别于瘢痕制备3天后局部应用相应药物,2周后取材,分别测量瘢痕组织TGF-β1mRNA的表达量。结果:积雪草甙组与空白对照组相比,TGF-β1mRNA表达量明显降低,有明显统计学差异(P<0.01);积雪草甙与阳性对照组相比,二者mRNA表达量无统计学差异(P>0.05);积雪草甙组之间,高浓度组与低浓度组相比有统计学差异(P<0.05)。结论:较高浓度积雪草甙注射液局部注射可以减少增生性瘢痕TGF-β1mRNA的表达。更多还原

【Abstract】 Objective To investigate the effects of Asiaticoside on TGF -β1 mRNA expression in hypertrophic scars.Methods Ninety-three full-thickness round wounds(1cm in diameter on ventral side of rabbits ears)on rabbits’ ears of 20 rabbits(four died)were created.Then rabbits were randomized into 4 groups:negative group,diprospan group,asiaticoside(25mg/ml)group and asiaticoside(50mg/ml)group.The corresponding injectant were given three days after the scar model was formed lasting for 7days.Samples were collected 2 weeks later and expressions of TGF-β1 mRNA of different groups were detected.Results Asiaticoside group has a lower expression of TGF-β1 mRNA compared with negative group(P<0.01),while there are no respective diversity(P>0.05)between asiaticoside group and masculine group.As to the two asiaticoside groups,group of higher concentration has a lower expression of TGF-β1 mRNA and the difference is significant.Conclusion Higher concentration of asiaticoside injected could inhibit the expression of TGF-β1 mRNA更多还原