【摘要】 使用马铃薯的偏爱密码子设计合成人胰岛素原基因,以植物表达载体pCAMBIA1301为基础,构建由patatin启动子调控的人胰岛素原基因植物表达载体p1301Ph,并将导入根癌农杆菌LBA4404中,经PCR鉴定证明,此基因已整合到农杆菌Ti上。表达载体的建立为日后转基因工作奠定了基础。更多还原

【Abstract】 Human proinsulin gene was designed and synthesized with the potato partial codon.Plant expression vector p1301Ph was derived from pCAMBIA1301,with human proinsulin gene being controlled by patatin promoter.Then the recombination plasmid was introduced into Agrobacterium LBA4404.It was proven that the vector was integrated into LBA4404.This study provides a foundation for the transgenic of plants.更多还原