【摘要】 2006-2007年,采用4个转CpTI基因棉花材料及其受体研究了转CpTI基因对棉花苗病、黄萎病、后期叶斑病及早衰的影响。结果表明:3个转CpTI基因材料苗病死苗率均低于其对应的受体材料。在6月底和7月初黄萎病的发生初期,转基因材料黄萎病发生较受体材料轻,7月中旬到8月底9月初,不同品种以及同一品种的不同生育期之间差异较大,转基因材料C连续两年病指极显著低于对照受体材料。与非转基因材料相比,转CpTI基因材料叶斑病的发生显著加重,早衰发生也加重。更多还原

【Abstract】 The cowpea trypsin inhibitor gene(CpTI)has been widely used in genetic engineering for more than ten years and has been successfully transferred into cotton(Gossypium hirsutum L.).Widely growing of transgenic plant may be accompanied by ecological effects including changes in diseases those coursed by fungus,bacterial or virus.People have paid more attention on environment risk assessment of transgenic plant recently.Most research on Bt cotton has focused on invasiveness,gene flow to indigenous organisms,development of resistant in target pests,and direct or indirect effects on non-target organisms and ecosystem.But a few studies have been published to evaluate the effects of CpTI on cotton diseases.Four CpTI cotton lines and their contrasts(non-CpTI cotton)were monitored for changes in diseases including seedling disease,Verticillium wilt,latter leaf spot and early senescence in 2006 and 2007.The results showed that the percentage of died seedling coursed by seedling disease of three CpTI cotton lines were lower than that of their contrasts(non-CpTI cotton).Verticillium wilt disease index(ID)of four CpTI cotton lines were lower than that of non-CpTI cotton lines in the end of June and the beginning of July(the beginning stage of Verticillium wilt),after that changes of Verticillium wilt ID were different among lines and stages.Verticillium wilt ID of CpTI A was remarkably higher than that of its contrast.ID of latter leaf spot and early senescence of four CpTI cotton lines were distinctly higher than that of four non-CpTI cotton lines.更多还原