【摘要】 利用RT-PCR和RACE技术从斑茅(Erianthus arundinaceus)中分离出编码鸟氨酸-δ-氨基转氨酶基因的全长cDNA序列,序列全长1 680 bp,编码454个氨基酸。通过对哺乳动物、高等植物、微生物的-δOAT基因编码的氨基酸序列进行同源比对,发现斑茅-δOAT基因同其近缘属植物甘蔗的同源性最高(87%),同其他高等植物的同源性次之(约为70%),而同动物的同源性最低(约为60%)。在斑茅-δOAT基因编码的氨基酸序列的5′端未发现线粒体定位序列,同甘蔗-δOAT基因一样。斑茅-δOAT基因具有完整的鸟氨酸转氨酶功能区rocD。利用定量RCR(real-tim e PCR)对30%PEG胁迫下的斑茅-δOAT基因表达量进行研究,结果表明-δOAT基因在胁迫12 h表达量达到最高,约为对照的4.1倍;胁迫2 h-δOAT基因表达量反而有所降低。更多还原

【Abstract】 The complete cDNA sequence of orn-δ-aminotransferase(OAT) gene was obtained from Erianthus arundinaceus and was cloned by reverse-transcript-polymerase-chain-reaction(RT-PCR) and rapid amplification of cDNA end(RACE) technologies.The acquired gene was 1 680 bp in full length,encoding 454 amino acid residues.The amino acid sequence blast results showed that compared to that from mammal,higher plant and microorganism,δ-OAT gene from E.arundinaceus shared the highest homology(87%) with relative genera plant,Saccharum officinarum,70% homology with other higher plants and 60% homology with animal.No N-terminal mitochondrial transit peptide(MTP) was found in the amino acid sequence encoded by δ-OAT gene from E.arundinaceus,which was the same as that from S.officinarum.Complete domain of OAT,rocD,was included in δ-OAT gene from E.arundinaceus.Expression level of δ-OAT gene from E.arundinaceus treated with 30% polyethylene glycol(PEG) was studied using real-time PCR technology,which showed that after treated 12 hours with PEG,the expression level reached the highest,4.1 times as that of the comparison,but got lower after stressed 2 hours.更多还原