【摘要】 以珙桐冬芽为材料进行组织培养和植株再生研究,结果表明:珙桐冬芽直接诱导丛生芽的最适培养基为WPM+NAA 0.2mg·L-1+6-BA 3.0mg·L-1+AC 2.0g·L-1;珙桐带芽茎段增殖的适宜培养基为WPM+NAA 0.05mg·L-1+6-BA 1.0mg·L-1+GA 32.0mg·L-1+AC 2.0g·L-1;生根最佳培养基为White+IBA 3.0mg·L-1+6-BA 1.0mg·L-1+AC 2.0g·L-1,在此条件下,根发育良好,植株健壮;组培苗炼苗后移栽,成活率可达80%。更多还原

【Abstract】 Using winter bud of Davidia involucrate as explant, we studied its tissue culture and plant regeneration. The results indicated: the best medium for the clump shoot induction directly from D. involucrata winter bud was WPM+NAA 0.2mg·L-1+6-BA 3.0mg·L-1+AC 2.0g·L-1; the appropriate medium for shoot segment multiplying of D. involucrate bud was WPM+NAA 0.05mg·L-1+6-BA 1.0mg·L-1+GA3 2.0mg·L-1+AC 2.0g·L-1; the medium for rooting culture was White+IBA3.0mg·L-1+6-BA 1.0mg·L-1+AC 2.0g·L-1, under these conditions, the roots were well developed and the plants were healthy; finally, about 80% of these seedlings could survival after transplantation.更多还原