【摘要】 目的观察大鼠脊髓缺血期间椎管内给予三磷酸腺苷对脊髓缺血再灌注损伤的作用。方法Wistar大鼠随机分为三磷酸腺苷组、生理盐水组、空白对照组。建立大鼠缺血再灌注模型,通过神经功能运动行为学和组织学评价大鼠脊髓缺血再灌注损伤程度,一步法缺口末端标记法检测神经元凋亡情况。结果三磷酸腺苷组大鼠运动行为学和组织学变化明显优于对照组,神经元凋亡数少于对照组。结论大鼠脊髓缺血期间椎管内给予三磷酸腺苷可以明显减轻脊髓缺血再灌注损伤,改善运动功能,减少脊髓神经元凋亡和坏死。更多还原

【Abstract】 Objective To investigate the effect of extraceullar adenosine triphosphate(ATP) injected into cavitas subarachnoidealis during ischemia on spinal cord injury following ischemiareperfusion. Methods Wistar rats were divided into three groups randomly:ATP group,normal saline group and control group.The spinal cord ischemia-reperfusion model was made and the injury degree of spinal cord was evaluated by footprint pattern test and histopathology and the apoptosis of neuron were evaluated by TdT-mediated dUTP nick end labeling.Results The motion function of spinal cord was improved significantly after treatment of ATP.ATP attenuated the neuron injury after spinal cord ischemic-reperfusion and the number of apoptosis of the anterior horn cells of spinal cord in the ATP group was decreased compared with the control group.Conclusion The motion function and apoptosis expression after spinal cord injury resulted from ischemia-reperfusion are attenuated significantly after treatment of ATP.更多还原