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淫羊藿药材的指纹图谱
Fingerprint of Medicinal Materials Epimedium
【摘要】 目的:建立淫羊藿药材的高效液相指纹图谱分析方法。方法:采用Agilent ODS-C18(4.6mm×150mm,5μm)色谱柱,柱温:40℃,流速:1mL/min。以乙腈、水为流动相进行梯度洗脱,检测波长为270nm;以淫羊藿苷为参照物。结果:通过对8个不同产地的淫羊藿药材样品的测定,标定了10个共有峰特征,并通过"中国色谱指纹图谱相似度评价系统2004A版软件",计算出其相似度。结论:该方法可为更好的控制淫羊藿药材的内在质量提供科学依据。
【Abstract】 Objective:To establish a high pressure liquid chromatography fingerprint analysis. Methods:Agilent ODS-C18(4.6mm×150mm,5μm) column,column temperature:40℃,flow:1mL/min. Acetonitrile and water as the mobile phase for gradient elution,detection wavelength 270nm; Icariin was used for reference. Results:Through the eight different samples of herbal origin determination Icariin,calibration of the peak total of 10 features,and through the "China chromatographic fingerprint 2004A similarity evaluation system software",to calculate their similarity. Conclusion:This method can be used to better control the quality of the internal medicine Icariin provide a scientific basis.
【Key words】 Epimedium; HPLC fingerprint; High pressure liquid chromatography;
- 【文献出处】 辽宁中医药大学学报 ,Journal of Liaoning University of Traditional Chinese Medicine , 编辑部邮箱 ,2009年05期
- 【分类号】R284.1
- 【被引频次】8
- 【下载频次】340