【摘要】 编码18-5.8-25S核糖体RNA的45S rDNA基因,是1个简单的多基因家族基因,一般以串联方式相连,对应于核仁组织区(NOR)。首次利用荧光原位杂交技术(FISH),研究45S rDNA在散生竹类的毛竹和斑竹,混生竹类的茶秆竹、日本矮竹、菲白竹和铺地竹,以及丛生竹类的白绿竹染色体上的分布。结果表明:本研究所涉及的散生竹和混生竹的几个竹种,只观察到1对随体染色体的次缢痕区域有45S rDNA位点,而丛生竹类的白绿竹除随体染色体具有45S rDNA位点外,某些非随体染色体上也有不同拷贝数的45SrDNA位点存在。更多还原

【Abstract】 Fluorescence in situ hybridization(FISH)was first applied in locating 45S rDNA sequence on metaphase chromosomes of Phyllostachys edulis,Ph.bambusoides f.lacrima-deae,Pseudosasa amabilis,Sasa fortunei,S.pygmea,S.argenteastriatus and Bambusa oldhamii.The 45S rDNA locus was detected in the secondary chromosomal constriction region for all the bamboo species investigated.Additional 45S rDNA loci were also found in non-satellite chromosomes in a sympodial bamboo species-B.oldhamii.更多还原